This kit provides everything required to isolate and purify inclusion body Proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilisation Reagent. Inclusion body Proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting. This kit provides a convenient way to screen recombinants prior to scaling up.
The process is efficient and streamlined and can process up to 12 samples in only 60 minutes. Each spin column is able to recover up to 50 µg of acidic or basic Proteins. Purified recombinant Proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.
ProteoSpin™ Inclusion Body Protein Isolation Micro Kit – 10300
Specification
| P4-0020 Specification 10300 |
Figure
Documentation
Literature
| P4-0020 Literature 10300 |
Protocol
| P4-0020 Protocol 10300 |
Manual
Safety Report
MSDS
| P4-0020 MSDS 10300 |
Related Products
| Catalogue Number | Description | Pack Size | Price | |
|---|---|---|---|---|
| P8-0052 | FastGene Scriptase Basic cDNA Synthesis Kit | 100 Reactions | £271.50 | View Product |
| P8-0054 | FastGene Scriptase II cDNA Synthesis Kit | 100 Reactions | £394.00 | View Product |
| P8-0056 | FastGene® 2x IC Green Universal qPCR Mix with Separate ROX | 100 Reactions | £52.00 | View Product |
| P8-0062 | FastGene® 2x IC Green Universal qPCR Mix (Fluorescein) | 100 Reactions | £52.00 | View Product |
| P8-0068 | FastGene® 2x PROBE Universal qPCR Mix with Separate ROX | 100 Reactions | £52.00 | View Product |
| P4-0168 | RNA/DNA Purification Kit - 48700 | 50 Preps | £775.00 | View Product |
| P4-0174 | RNA/DNA/Protein Purification Plus Micro Kit - 51600 | 50 Preps | £935.00 | View Product |
| P4-0019 | RNA/Protein Purification Plus Kit - 48200 | 50 Preps | £716.00 | View Product |
| P4-0090 | RNA Clean-Up & Concentration Kit - 23600 | 50 Preps | £309.00 | View Product |
| P4-0091 | RNA Clean-Up & Concentration Kit, 96 Well Format - 25100 | 2 Plates | £918.00 | View Product |
| P4-0015 | Total RNA Purification Plus Kit - 48300 | 50 Preps | £445.00 | View Product |
| P4-0016 | Total RNA Purification Plus Kit - 48400 | 100 Preps | £685.00 | View Product |
| P4-0057 | Total RNA Purification Maxi Kit - 26800 | 8 Preps | £201.00 | View Product |
| P4-0058 | Total RNA Purification Kit - 17200 | 50 Preps | £329.00 | View Product |
| P4-0059 | Total RNA Purification Kit, 96-Well Kit - 24300 | 2 Plates | £815.00 | View Product |
| P4-0164 | Single Cell RNA Purification Kit - 51800 | 50 Preps | £542.00 | View Product |
| P4-0336 | Total RNA Purification Plus Micro Kit - 48500 | 50 Preps | £513.00 | View Product |
| P4-0426 | Total RNA Purification Kit - 17270 | 500 Preps | £2,449.00 | View Product |
| P4-0442 | Total RNA Purification Kit, 96-Well Kit - 24370 | 6 Plates | £2,320.00 | View Product |
| P4-0440 | Total RNA Purification Kit, 96-Well Kit (Deep Well Format) - 24350 | 2 Plates | £815.00 | View Product |
| P4-0444 | Total RNA Purification Kit, 96-Well Kit (Deep Well Format) - 24380 | 6 Plates | £2,320.00 | View Product |
Accessories
No accessories products found.